Question 1 In the initiation of translation in eukaryotes, the ______ ribosomal subunit binds near the 5' end of the mRNA and locates the ______ sequence to find the start codon.
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Adi S.
Q1: On the ribosome, the mRNA is read from 5' to 3' in a series of three nucleotides called codons. Q2: Which of the following features of eukaryotic ribosomes in translation initiation is FALSE? a. It uses an initiator tRNA that carries a methionine. b. The large subunit of the ribosome is important for binding to the mRNA. c. The large and small subunits of the ribosome combine once the initiation complex recognizes the AUG start codon. Q3: Which of the following methods is NOT used by eukaryotic cells to regulate the amount of protein in the cell? a. Operons b. Riboswitches c. miRNAs
Md.Daniyal A.
Answer ALL of the following parts: Question 2: A) In prokaryotes, a small ribosomal subunit can potentially get on an mRNA anywhere it can find enough space to do so. Once a small ribosomal subunit has bound to an mRNA, it will scan along that mRNA in the 5' to 3' direction looking for a start codon at which to initiate translation. How does the small ribosomal subunit distinguish a start codon from any other AUG codon that simply codes for methionine in the middle of a coding sequence? B) Prokaryotic cells can and do produce "polycistronic" mRNAs, which have multiple independent coding sequences coding for separate proteins on the same mRNA strand. Eukaryotic cells don't have polycistronic mRNAs, because only the first (most 5') coding sequence on such an mRNA would ever be translated in a eukaryotic cell. Explain why this is the case - why wouldn't a second, more 3' coding sequence on an mRNA be translated in a eukaryotic cell?
Jil M.
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