In a eukaryotic cell, how would each of the following affect gene expression? removal of nucleosomes from the DNA addition of a methyl group to the DNA binding of a general transcription factor to an enhancer region mutation of the TATA box binding of a repressor to the gene promoter loss of the mRNA poly-A tail binding of a RBP to increase mRNA stability binding of a RISC to mRNA addition of a ubiquitin to the protein product
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Gene expression is the process by which information from a gene is used to synthesize a functional gene product, usually proteins, through the processes of transcription and translation. This process can be regulated at various stages, including chromatin Show more…
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The following are examples of how eukaryotic cells may control gene expression: small RNA molecules, methylation of DNA and histones, histone acetylation, promoter region that controls the initiation of transcription, enhancer regions which are frequently found very far away from the promoter region, alternative splicing of mRNA, RNA binding proteins, 5' cap, and 3' poly-A tail on mRNA to improve stability and reduce likelihood of degradation, and inducible and repressible operons.
Shaiju T.
What type of RNA processing occurs at the 5' end of mRNA molecules in eukaryotic cells? Addition of a guanine nucleotide Addition of up to 250 adenine nucleotides Removal of introns addition of 7MG Genes in eukaryotic cells often have intronic sequences coded for within the DNA. These sequences are ultimately not translated into proteins. Why? Intronic sequences are removed from RNA molecules by the spliceosome, which works in the nucleus. Introns are not transcribed by RNA polymerase. Introns are removed by catalytic RNAs in the cytoplasm. The ribosome will skip over intron sequences when translating RNA into protein.
Shyam P.
You are studying the rate of transcription of a particular eukaryotic gene. When the DNA located several thousand bases upstream from the gene is removed, the transcription rate of the gene decreases dramatically. How would you interpret these results?
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