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Cholesterol quantification using gas chromatography, gc, and liquid chromatography, lc, involves several steps.
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One, sample preparation.
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So the first step is to prepare the sample.
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This involves extracting the cholesterol from the sample, which can be done using a solvent like chloroform or methanol.
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The sample is then dried and reconstituted in a suitable solvent for the chromatography process.
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Two, derivatization.
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In gas chromatography, cholesterol is often derived to make it more volatile and thus more amenable to gc analysis.
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This is typically done using a reagent like bstfa, which converts the cholesterol into a trimethyl derivative.
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Three, chromatography.
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The sample is then injected into the chromatography system.
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In gc, the sample is vaporized and carried by an inert gas like helium through a column...