question 2 an enzyme has been purified from ian comc up with comniel mecnanismn ietrovirus which

1. The enzyme ARVIZ cleaves before large aliphatic residues in the peptide LRDL.

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Question 2: An enzyme has been purified from an oncogenic retrovirus which you call ARVIZ. You need help developing a drug to combat this pathogen. Here is what it is known to cleave: large, aliphatic residues. The following have no effect on its activity: DIFP, IOAc. Addition of EDTA followed by dialysis, and then reconstitution with ions has the effect shown on the left following treatment: Treatment No treatment EDTA dialysis then add H2O then add Mg2+ then add Co2+ then add Fe2+ ACvin Ioc 1 1 It has the pH profile shown on the right. From these data, propose a complete mechanism for the action of ARVIZ on the peptide LRDL. Include the following: Substrates, Products, Transition states and Intermediates (if any), Arrows showing electron movement, and how bonds get made and broken. Amino acids and other structural elements necessary for catalysis; be specific. The role that each structural element plays in the catalysis, and which tool from that catalysis toolbox. Where could spin-labeled H2O be incorporated?

          Question 2: An enzyme has been purified from an oncogenic retrovirus which you call ARVIZ. You need help developing a drug to combat this pathogen. Here is what it is known to cleave: large, aliphatic residues. The following have no effect on its activity: DIFP, IOAc. Addition of EDTA followed by dialysis, and then reconstitution with ions has the effect shown on the left following treatment:

Treatment No treatment EDTA dialysis then add H2O then add Mg2+ then add Co2+ then add Fe2+
ACvin
Ioc
1 1

It has the pH profile shown on the right.

From these data, propose a complete mechanism for the action of ARVIZ on the peptide LRDL. Include the following: Substrates, Products, Transition states and Intermediates (if any), Arrows showing electron movement, and how bonds get made and broken. Amino acids and other structural elements necessary for catalysis; be specific. The role that each structural element plays in the catalysis, and which tool from that catalysis toolbox. Where could spin-labeled H2O be incorporated?

Added by Taylor R.

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