00:01
Okay, i want to answer this question, let's talk about the electron transfer chain.
00:03
It says, electron transfer chain from support on grading across the inner mitochondrial membrane, which drives the synthesis of atp via chemoctmosis.
00:11
So let's talk about how atp is produced via the electron chain.
00:17
If this is your outer mitochondrial membrane and this is your inner mitochondrial membrane, here you're going to have the electron transfer chain that has complex number one, two, three, and four.
00:25
During metabolism, you're going to produce electron carriers that are in adhd and fadh2.
00:32
And they are going to donate electrons to compress number one and to compress number two, respectively.
00:36
Then these electrons are going to flow to complex number three, and then to compress number four, where oxygen is going to accept those electrons.
00:44
The goal of all of this is to pump protons by complex number one, by complex number three, and by complex number four, from this space that is a mitochondrial matrix to this space that is an intermembronous space.
00:54
Okay? so you're going to have, you're going to gain protons here, and you're going to lose protons here.
00:59
So you're going to have a gradient because of this flow of electrons.
01:02
Across the eleon transport chain.
01:05
So as you are going to have a gradient, then these protons are going to want to move back to the matrix.
01:09
But the problem is that this inner mitochondal membrane is not permeable to protons.
01:14
So the only way by which they can come back to the matrix is by a transporter that is here that is called atp synthase.
01:22
So this atp synthase is going to allow, it is going to allow protons to move back to the matrix.
01:28
And as it does this process, it is going to phosphorylate adp and it is going to produce, this is how atp is produced via all of this process.
01:38
This question says, the question is a question is, 2 -4, the nitrophenol is a type of proton ionophores which function as an coupling, uncapling agent.
01:46
Uncapling agents disgrouped the proton -motive force, explain the electron transport chain, and discuss how this drag affects the electron transport chain the overall aerobic expiration process.
01:55
Okay, so i have already explained the process of the electron transfer chain.
01:59
Now i have to tell you how this dnp is going to affect the electron transport chain and also the aerobic respiration process.
02:07
What this dmp is going to do is that it is going to bind to these protons hitting the interminibrandous space and it is going to transport them across the inner mitochondal membrane to the mitochondrial matrix.
02:19
So practically it is going to help protons to move back to the mitochondal matrix without using this atp synthase.
02:26
So as protons are going to come back to the mitochondal matrix without using this atp synthase, then this atp synthesis is not going to move protons back to the matrix.
02:35
And as it is not going to move protons back to the matrix, then it is not going to rotate and it is not going to phosphorylate adp, and hence it is not going to produce atp.
02:46
So what you're going to have is that you're going to have low levels of atp because of this dmp.
02:52
So as you can see here, as all of your electrical gradient of protons is going to be dissipated, then your metabolism is going to increase.
03:01
You're going to have an increased metabolism, and this is why this drug was used to lose weight, because the metabolism is going to increase in order to produce more an adhd and fadh2 in an attempt to produce more atp.
03:17
But this is not going to work because all of the proton gradient that you're producing here is going to dissipate, and it's going to be lost as heat, because this inner mitochondal membrane is going to be, it's not going to be like impermeable to protons anymore because the nep is transporting these protons back to the matrix.
03:34
So all of this is going to be disrupted.
03:36
Now let's hope for question b that says show a detailed calculation of atp synthesized by oxidative fluoride if five molecules of glucose completely oxidized during cellular respiration process...