Question 3 We will purify your PCR products and evaluate their quality using spectrophotometry. What wavelength of light is absorbed by DNA, but not other macromolecules like proteins? A 260nm B 280nm C 410nm D 600nm 1 Point
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Step 1: The question asks for the wavelength of light absorbed by DNA but not by proteins, which is a common method to assess DNA purity using spectrophotometry. Show more…
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2) Measuring the intensity of absorbance of the DNA solution at wavelength 260nm & 280nm is used as a measure of DNA purity: At what A260/A280 ratio, it shows good DNA purity? A) 0.6 B) 1.0 C) 1.4 D) 1.8 3) Which below cannot precipitate protein in order for DNA to be separated by salting out?
Adi S.
Question #1 Which of the following wavelengths of light is DNA best at absorbing? A. 220 nm B. 590 nm C. 360 nm D. 260 nm
You have purified DNA into a final volume of 0.5 mL (Tube A). From this, 20 µL was placed into a microcentrifuge tube and topped up to 1 mL of water (Tube B). You then placed the diluted contents into a 1 cm cuvette and obtained the following spectrum: What is the concentration of DNA in Tube A? Select one: a. 1250 µg/mL b. 2000 µg/mL c. 875 µg/mL d. 3500 µg/mL e. 1750 µg/mL
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