Order the following steps required for DNA synthesis according to their decreasing relative energetic costs associated with replicating a genome. DNA priming and primer removal (assume 20 ATP equivalents per RNA primer of 10 nts) Ligation of Okazaki fragments (2 ATP equivalents per DNA ligase reaction) de novo synthesis of purine dNTP molecules required to replicate the genome. de novo synthesis of pyrimidine dNTP molecules required to replicate the genome. DNA unwinding (2 ATP equivalents per 2 bp advance of DNA helicase) DNA polymerization (2 ATP equivalents per incorporated dNTP by DNA polymerase)
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De novo synthesis of purine dNTP molecules required to replicate the genome: Purine synthesis requires a significant amount of energy. To synthesize one purine dNTP, it takes around 20 ATP equivalents. Show more…
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Put the events of DNA replication in the correct order. One strand is synthesized continuously, while the other is formed in pieces. A primer RNA sequence is added to the DNA strand. RNA primers are removed and replaced with DNA nucleotides and ligase seals the backbone of the DNA. Helicase unwinds the DNA strand.
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