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Question 4) The gene X you are studying has 3 exons and 2 introns with the following sizes: size exon 1: 2 kb exon 2: 1 kb exon 3: 3 kb intron 1: 2 kb intron 2: 3.5 kb 4.1. Draw a diagram of the DNA using the line below. Below your diagram, draw the mature, processed mRNA (roughly to scale). Where appropriate, indicate the location of each intron and exon, the 5'UTR, the 3'UTR, the 5' cap, the start codon, the stop codon, the polyA tail, splicing regulatory sequences. ______________________________________________________________________________________________________ 4.2. In a study investigating changes induced by RNA splicing mutations, you suspect that gene X is mis-spliced in the patient samples. The sequencing results predict that there are two independent alterations: (i) a retention of intron 1 and (ii) a skipping of exon 2. You are tasked with designing primer pairs to run a quantitative RT-PCR (to yield an amplicon product of about 100-150 bp in length) to distinguish these 2 abnormal transcripts from the normal mature transcript (3 exons). How will you design these primer pairs? Draw the scheme with locations of the primers along gene X.

          Question 4) The gene X you are studying has 3 exons and 2 introns with the following sizes:

          size
exon 1: 2 kb
exon 2: 1 kb
exon 3: 3 kb
intron 1: 2 kb
intron 2: 3.5 kb

4.1. Draw a diagram of the DNA using the line below. Below your diagram, draw the mature, processed mRNA (roughly to scale). Where appropriate, indicate the location of each intron and exon, the 5'UTR, the 3'UTR, the 5' cap, the start codon, the stop codon, the polyA tail, splicing regulatory sequences.

______________________________________________________________________________________________________

4.2. In a study investigating changes induced by RNA splicing mutations, you suspect that gene X is mis-spliced in the patient samples. The sequencing results predict that there are two independent alterations: (i) a retention of intron 1 and (ii) a skipping of exon 2. You are tasked with designing primer pairs to run a quantitative RT-PCR (to yield an amplicon product of about 100-150 bp in length) to distinguish these 2 abnormal transcripts from the normal mature transcript (3 exons). How will you design these primer pairs? Draw the scheme with locations of the primers along gene X.

Added by Jose Luis L.

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