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Question 4 [total mark value: 20] Next, you take a culture of the bacterial isolate growing in rich media. You split the culture into two portions. One portion you continue to grow at 25°C, while the other you put to grow at 37°C. After 30 minutes, you look at the bacteria under the microscope. You notice that at 37°C, almost none of the bacteria are motile. After another hour, the bacteria start forming a thick layer of biofilm at the liquid-air interface and on the surface of the glass culture tube. What conditions does growing the bacteria at different temperatures emulate? (6 marks) Provide an explanation for why the bacteria stop being motile? (7 marks) Provide an explanation for why the bacteria start forming biofilm? (7 marks)

          Question 4 [total mark value: 20]
Next, you take a culture of the bacterial isolate growing in rich media. You split the culture into
two portions. One portion you continue to grow at 25°C, while the other you put to grow at
37°C. After 30 minutes, you look at the bacteria under the microscope. You notice that at 37°C,
almost none of the bacteria are motile. After another hour, the bacteria start forming a thick
layer of biofilm at the liquid-air interface and on the surface of the glass culture tube.
What conditions does growing the bacteria at different temperatures emulate? (6 marks)
Provide an explanation for why the bacteria stop being motile? (7 marks)
Provide an explanation for why the bacteria start forming biofilm? (7 marks)

Question 4 [total mark value: 20]
Next, you take a culture of the bacterial isolate growing in rich media. You split the culture into
two portions. One portion you continue to grow at 25°C, while the other you put to grow at
37°C. After 30 minutes, you look at the bacteria under the microscope. You notice that at 37°C,
almost none of the bacteria are motile. After another hour, the bacteria start forming a thick
layer of biofilm at the liquid-air interface and on the surface of the glass culture tube.
What conditions does growing the bacteria at different temperatures emulate? (6 marks)
Provide an explanation for why the bacteria stop being motile? (7 marks)
Provide an explanation for why the bacteria start forming biofilm? (7 marks)

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