00:01
Okay, so this question is asking all about endospores.
00:05
And so which of the following statements is true? so the first statement, endospores are easily stained in a gram stain that is false.
00:13
Endospores are not.
00:14
So let's actually discuss what an endosporus.
00:16
So we have endospores are what is the precursor to essentially spores.
00:24
So we have these typically bacilli rods, not the genus basilis basilis, but the rod -shaped bacteria are going to be your spore -forming, or if they are a spore -forming, they will be a rod -shaped bacteria.
00:40
They will have something during the process of sporyulation.
00:44
They will create, they'll be in a state known as, not a state, but they will, in the process of sporyulations, in the process of making a spore, they will create what's known as an endospore, which is this guy right here.
01:03
And then that endospore is eventually released to become an actual bacterial spore.
01:10
Endospore, endo means in, so an endospore is when the spore is inside the broad -shaped bacteria.
01:15
So this is the mother's cell.
01:18
In this state, this is known as the mother's cell.
01:29
Now, if this endospor didn't exist, you will have just a broad -shaped bacteria, which will be known as a vegetative cell.
01:38
But we do have an endospor, and that's what this.
01:43
Here.
01:45
Typically, sometimes actually, you can see the endospor forming at near a clubbed end right here because the endospor is getting larger, so one end may be a bit larger.
01:59
But actually, if you're looking at it under a gram stain, you can sometimes even tell there is an endospor.
02:08
Because the endospore itself will not stain.
02:13
It has layers upon layers of protenacious coats.
02:19
And so that part will not stain.
02:21
But the rest of the cell can actually stain.
02:27
So you'll have your gram stain will look something like this.
02:34
Okay.
02:35
So those bacterial cell itself, the mother cell will get stained, but the endospore will not get stained.
02:58
So going through, first statement, endospores are easily stained and a gram stain.
03:03
They are not stained.
03:05
You can potentially tell if a cell has endospores because of this phenotype that i just showed here, that drew here, but it is not necessarily, but it is not because you are staining the endospore.
03:19
The endospore remains unstained...