Question 46 The purpose of adding arabinose to the media is to: Group of answer choices A. enable the RNA polymerase to bind to the operator B. allow the ampicillin to aid in bacterial production of huge quantities of GFP C. continue DNA replication and transcription to make RNAs to produce GFP D. induce the operon to produce the GFP protein Question 47 Why was the plasmid not added to the -pGLO LB plate? Group of answer choices A. the bacteria is being monitored to determine if the ampicillin gene is present B. the plasmid is being checked to determine if the GFP gene is present C. the plasmid was added in a prior step D. the -pGLO LB plate is a control to confirm that bacteria is viable and growing Question 48 If bacteria that did not receive a plasmid are placed on an LB plate with ampicillin, what would you expect to happen? Group of answer choices A. only a few bacterial colonies will grow B. the bacteria will create a lawn C. the bacteria will not grow D. the bacteria will glow a green color under UV light Question 49 The purpose of the agar plate with +pGLO/LB/amp is to: Group of answer choices A. destroy extra plasmids B. kill all bacteria so the dish is sterile C. fight off invading bacteria D. identify transformed bacteria Question 50 In the GFP lab experiment, which of the following will fluoresce under UV light? Group of answer choices A. E. coli (-pGLO) on LB amp plate B. E. coli (+pGLO) on LB amp plate C. E. coli (+pGLO) on LB amp+ara plate D. E. coli (-pGLO) on LB plate Question 51 Which of the following would not be a valid precaution when using a tissue homogenizer? Group of answer choices A. make sure to place the top securely on the rotor B. take care to not touch the lower blades while they are moving to avoid being cut C. wear goggles to protect one's eyes D. turn the machine on and off while the probe is surrounded by liquid
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So the correct answer is: $\boxed{\text{D}}$ Question 47: The plasmid was not added to the -pGLO LB plate because the -pGLO LB plate is a control to confirm that bacteria is viable and growing. So the correct answer is: $\boxed{\text{D}}$ Question 48: If Show more…
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