Question 5 1 pts We learned about the Trp repressor's regulation of the trp operon in E. coli. The Trp repressor undergoes a conformational shift in the presence of tryptophan, which changes the configuration of the helix-turn-helix domain to allow it to bind to DNA. What is this phenomenon an example of? Phosphorylation-dependent activity Transcription factor cooperativity Post-translational chemical modification Allosteric regulation
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The trp repressor plays a role in regulating the tryptophan operon. The trp repressor is best characterized by which of the processes in the figure? Ligand-Regulated Repression Ligand-Regulated De-repression Ligand-Regulated Activation Ligand-Regulated Deactivation
Adi S.
What molecule serves as a co-repressor for the trp operon? a) Ribosome c) trpR e) Glucose b) Tryptophan d) ̢-galactosidase 6. Which of the following statements about the "glucose effect" is true? a) The activity of adenylcyclase is inhibited by glucose. b) Cyclic AMP levels increase when glucose is present. c) CAP activates transcription of the lac operon when glucose is present. d) Cells will metabolize lactose when glucose is present. 7. Attenuation of the trp operon does not include which of the following? a) trpR c) ribosome e) all are needed b) tryptophan d) RNA polymerase 8. The lac operon, when using lacl, is regulated by a(n) a) activator and inducer c) activator and co-repressor b) repressor and inducer d) repressor and co-repressor 9. The trp operon is regulated by a(n) a) activator and inducer c) activator and co-repressor b) repressor and inducer d) repressor and co-repressor 10. Pairing of which sequence regions within trpL actually result in the transcriptional termination of the trp operon? a) 1 and 2 b) 2 and 3 c) 3 and 4 d) 1 and 4
Sri K.
Q5 What is the function of cAMP in the regulation of the lac operon? a. It activates a repressor protein b. It activates an activator protein c. It inactivates a repressor protein d. It inactivates an activator protein e. It causes attenuation Q6 Which of the following types of eukaryotic gene regulation is at the level of DNA? a. mRNA processing b. RNA interference c. Histone acetylation d. mRNA stability e. Post-translational modification Q7 If a mutation prevents the formation of the antiterminator 2+3 loop in the trp operon, what would be the effect? a. Transcription only when tryptophan is absent b. Constitutive attenuation of transcription c. Transcription only when tryptophan is present d. Constitutive transcription e. No effect, as 2+3 loop has no function Q8 Although operons are not common in eukaryotes, eukaryotic genes may be activated by the same stimulus. Which of the following DNA regulatory sequences makes this coordinated gene expression possible? a. Response element b. Core promoter c. Enhancer element d. Boundary element e. Silencer element Q9 Insulators can block the effects of enhancers only when they lie: a. Between an enhancer and a promoter b. Upstream of a promoter c. Adjacent to a promoter d. Within the structural genes e. Within a consensus sequence
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