QUESTION 7 A microscope that is not parfocal would be difficult to use since it can't reach high magnifications because the lenses are not compound. You would only be able to use one eye. You couldn't estimate the final focal plane at lower magnifications. Oil immersion would not be possible. All of the above are true. QUESTION 8 Immersion oil decreases reflection and refraction. Why does this help microscopy? Higher magnification levels don't refract light. Higher magnification levels need more light. Lower magnification levels don't have any reflection and refraction. Oil immersion would not be possible at lower magnification levels. All of the above are true. QUESTION 9 Using the 40X objective lens on the compound microscope with a 20X ocular lens gets what final magnification? 2x. 60x. 80x. 800x. 6000x.
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You also couldn't estimate the final focal plane at lower magnifications, and oil immersion would not be possible. Therefore, all of the above statements are true. Show more…
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Which of the following changes would increase resolution when using the oil immersion lens? Choose all that are correct. a. Adjust fine focus knob. b. Use immersion oil. c. Increase rheostat intensity. d. Raise condenser. e. Lower condenser. f. Open iris diaphragm. g. Close iris diaphragm. Answer choices: A. a, c, e, f B. a, b, e, g C. a, b, c, d, f D. b, c, d, e 2. Why must immersion oil be used with the oil immersion lens? A. To increase contrast. B. To increase light refraction to decrease contrast. C. To decrease light refraction thus increasing resolution. D. To increase light refraction to increase resolution. 3. To focus a specimen, it is best to start with the ___________ objective lens. A. Oil immersion. B. Ocular. C. High dry. D. Scanning. 4. What is the function of the iris diaphragm and substage condenser? A. To focus using oil immersion lens. B. To move slide left to right. C. To move stage up and down. D. When opened and raised, increases light focused on specimen thus increasing resolution. 5. When using the high dry or oil immersion lens, which focus adjustment knobs should never be used? A. Fine focus adjustment knob. B. Medium focus adjustment knob. C. Low focus adjustment knob. D. Coarse focus adjustment knob.
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1. If a specimen is viewed using a 45X objective in a microscope with a 15X eyepiece, how many times has the image been magnified 2. Would you use phase contrast microscopy to view a stained preparation? Would you use a coverslip on a stained preparation? 3. Would you use bright field microscopy to view a wet mount preparation? Would you use a coverslip on a wet mount preparation? 4. What is the position on the condenser wheel for (1) bright field – low 10X lens, (2) phase contrast – high dry (40X) lens, (3) phase contrast – oil immersion (100X) lens, and (4) bright field – oil immersion (100X) lens?
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Given two 12-cm-focal-length lenses, you attempt to make a crude microscope using them.While holding these lenses a distance 55 cm apart, you position your microscope so that its objective lens is distance from a small object. Assume your eye's near point $N =$ 25 cm. ($a$) For your microscope to function properly, what should $d_{\circ}$ be? ($b$) Assuming your eye is relaxed when using it, what magnification $M$ does your microscope achieve? ($c$) Since the length of your microscope is not much greater than the focal lengths of its lenses, the approximation $M\approx N \ell/f_ef_\circ$ is not valid. If you apply this approximation to your microscope, what % error do you make in your microscope's true magnification?
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