Question 7. Anfinsen studied the folding of ribonuclease in one of the landmark experiments in biology which won him the Nobel Prize in 1971.
A] Place the steps of Anfinsen's ribonuclease folding experiment in the correct order for the renaturation of the protein (all the
steps may not be necessary)
1) Treatment with HCI, II) Removal of urea, III) Removal of ẞ-mercaptoethanol, IV) Denatured protein, v) Native protein
B] The following statement is FALSE. Correct one or more of the scientific terms below so that they become true statements. (You cannot
simply make a negative sentence!)
In Anfinsen's experiment, the removal of beta-mercaptoethanol by dialysis resulted in formation of incorrect disulfide bonds.
C] True or False - If Anfinsen had used another enzyme HIV protease instead of ribonuclease in his experiment, he
would have only used urea since there are no disulfide bonds in HIV protease.