Question

You determine the sequence of an isolated gene, and wish to find its location in the genome. Propose a procedure using fluorescence to locate your gene of interest. Your Answer: 1. Synthesis of single-stranded probe molecule, which is complementary to your gene and labeled with a fluorescent marker 2. Immobilisation of mitotic cells 3. Denaturation of the chromosomes 4. Introduction of labeled probe 5. Hybridization 6. Detection of the fluorescent label 7. Identification of the chromosome and location of the gene

          You determine the sequence of an isolated gene, and wish to find its location in the genome. Propose a procedure using fluorescence to locate your gene of interest.

Your Answer:
1. Synthesis of single-stranded probe molecule, which is complementary to your gene and labeled with a fluorescent marker
2. Immobilisation of mitotic cells
3. Denaturation of the chromosomes
4. Introduction of labeled probe
5. Hybridization
6. Detection of the fluorescent label
7. Identification of the chromosome and location of the gene

You determine the sequence of an isolated gene, and wish to find its location in the genome. Propose a procedure using fluorescence to locate your gene of interest.

Your Answer:
1. Synthesis of single-stranded probe molecule, which is complementary to your gene and labeled with a fluorescent marker
2. Immobilisation of mitotic cells
3. Denaturation of the chromosomes
4. Introduction of labeled probe
5. Hybridization
6. Detection of the fluorescent label
7. Identification of the chromosome and location of the gene

Added by Juan Luis S.

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