Question 8 (1 point) Saved What particular feature of molecular hybridization makes it a powerful tool in molecular biology That molecular hybridization is highly specific None of these responses are correct The fact that DNA molecules can bind RNA molecules That hybridization occurs via typical base pairing interactions The denaturation is controlled by heat or chemical treatment
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1. DNA favors hybridization (formation of double-stranded DNA) due to like charge repulsion of backbone phosphate groups. 2. High GC content favors hybridization due to base stacking. 3. Base pairing favors hybridization due to entropy. 4. All of the above favor hybridization. 2. Which of the following is NOT a prediction that arises from Chargaff's rule? a. G and C base pair with each other, and A and T base pair with each other in DNA. b. DNA is antiparallel. c. # purines = # pyrimidines in DNA. d. DNA is double-stranded. 3. Which of the following is NOT true of B form nucleic acid duplexes? a. It is the form adopted by DNA. b. It is the form with the smaller diameter. c. It is the form with bases nearly perpendicular to the long axis. d. It is the form with the deep major groove. 4. True or False: The function of RNA depends on its sequence. Tertiary structure is not relevant to function. a. True b. False 5. DNA absorbs electromagnetic radiation maximally in the UV spectrum at ____________ nm, and this wavelength can therefore be used to quantify DNA concentration or melting temperature. This is as compared to protein, which absorbs maximally at ____________ nm due to its aromatic amino acid side chains. FILL IN THE BLANKS.
Md.Daniyal A.
Oligonucleotide probes can be used to rapidly detect the presence of a targeted mutant sequence. How does the probe know where to bind in the patient's genomic DNA? Binding between a nucleic acid and a DNA polymerase ensures that probes are sequence specific. Probes are carefully targeted to specific genes by hydrogen bonding. The greater the GC content of the genomic DNA, the stronger the targeting. DNA polymerase recognizes the short stretches of double stranded DNA formed by hybridization between the probe and a perfect match target binding site. This ensures that if the probe binds in the wrong place that DNA synthesis will not initiate. Oligonucleotide probes bind to their target sequence with an affinity that reflects nearest neighbor thermodynamic interactions. They don't really because the probes don't have brains. They just stick to any DNA molecule that has a fully complimentary sequence unless the assay temperature is below the Tm for a mismatch between the probe and a less-than-perfect binding site.
Adi S.
The "complementary sequence that is created to identify a particular stretch of DNA" is the: probe primer polymerase hybridization Dr. Brown obtains "intermediate" results from a susceptibility test. She may correctly interpret this to mean that the antimicrobial agent in question: may be successfully used to treat infection caused by the bacterial isolate test is potentially useful if the concentration is high enough cannot be effective against the tested isolate is not appropriate for treating infection caused by this bacterial isolate because the test highly correlates with a resistance mechanism that renders treatment success doubtful 1 and 4 The major advantage of microarrays is that they: allow the analysis of nucleic acids much more rapidly after performance of a Southern blot allow the separation of different fragments of nucleic acids according to their molecular size quantitate DNA synthesis in a sample in real time allow the simultaneous analysis of a very large number of target sequences
Asma V.
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