What is the purpose of the GFP gene in the pGLO plasmid? A. none of the above B. to maintain the integrity and structure of the pGLO plasmid when incorporated into the E. coli cells C. allows the GFP dye to trigger a color change in the competent E. coli cells D. this allows cells that have picked up the pGLO plasmid to fluoresce green when the gene is expressed
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On the pGLO plasmid, what is the bla gene for? Group of answer choices It is the origin of replication so the bacterial cell can copy the plasmid. It codes for the green fluorescent protein. It allows us to select for bacterial cells that picked up the plasmid. It allows us to control whether the GFP gene is expressed or not.
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Check all that apply. The pGLO plasmid is a recombinant plasmid that has which of the following genes? A. bla B. GFP C. lacZ D. araC 2. The following is true of the bla gene on the pGLO plasmid except A. confers antibiotic resistance towards ampicillin B. produces the beta-lactamase enzyme C. acts as a selectable marker D. encodes the regulator protein 3. The GFP gene A. glows green in the presence of arabinose and UV light B. has ampicillin resistance C. will not grow on plates with ampicillin D. contains the pGLO plasmid
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A geneticist uses a plasmid for cloning that has the lacZ gene and a gene that confers resistance to penicillin. The geneticist inserts a piece of foreign DNA into a restriction site that is located within the lacZ gene and uses the plasmid to transform bacteria. Explain how the geneticist can identify bacteria that contain a copy of a plasmid with the foreign DNA.
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