When inoculating the plate for the antimicrobial drug susceptibility disk diffusion assay, you streak _____ using the streak-plate method to obtain single colonies. all over the plate multiple times to obtain confluent growth all over the plate once to obtain confluent growth In the antimicrobial drug susceptibility disk diffusion assay, the antimicrobial drug concentration on an agar plate is lowest in the agar medium closest to the disk and increases as the drug moves away from the disk. highest in the agar medium closest to the disk and decreases as the drug moves away from the disk. the same throughout the agar medium.
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Matching Question Place the following steps for disk diffusion test in the right sequence. Make a cell suspension (test bacteria, reference bacteria) at 0.5 McFarland standard Apply antimicrobial-infused disks of interest Spread the suspension on an agar plate to make a lawn Measure the diameter of the zone of inhibition in mm Use the reference to determine sensitivity or resistance of the test bacterium to the drug Incubate the plate to allow for growth of the test bacterium and diffusion of the antimicrobial agent 1. Step 1 2. Step 2 3. Step 3 4. Step 4 5. Step 5 6. Step 6
Anand J.
You culture an inoculum of bacteria using the pour plate method and start by mixing 1 mL of the sample with 9 mL of sterile broth medium in a tube (tube 1). You then repeat this process to carry out serial 1/10 dilutions, each time mixing 1 ml of the diluted bacteria with 9 mL of medium (tubes 2, 3, 4, 5). You prepare pour plates by mixing 1.0 mL from each tube with 15 mL of cooled but still melted agar poured into a petri dish, and allow to solidify. You then incubate the plates for 24 hours and count the resulting colonies on each plate. If you obtain 70 colonies on plate 2 (prepared using the 2nd tube), how many bacteria were present in your original sample?
Sri K.
"In the laboratory, a sterile inoculating loop is moved across the agar surface in a culture dish, thinning a sample and isolating individuals." This statement describes which of the following? a. broth culture b. pour plate c. streak plate d. dilution plate
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