Questions for REVERSE-PHASE CHROMATOGRAPHY 1. What is the purpose of the reverse-phase chromatography experiment? 2. Which dye was collected first? Why? 3. What is the function of the alcohol? 4. Why are two different percentages of alcohol used? 5. What would have happened if you had eluted first with 35% alcohol? 6. Which fraction contained the sugar?
Added by Michele S.
Step 1
The purpose of reverse-phase chromatography is to separate and purify compounds based on their hydrophobicity. In this technique, a nonpolar stationary phase is used, and the mobile phase consists of a polar solvent. Show more…
Show all steps
Your feedback will help us improve your experience
Adi S and 98 other Biology educators are ready to help you.
Ask a new question
Labs
Want to see this concept in action?
Explore this concept interactively to see how it behaves as you change inputs.
Key Concepts
Recommended Videos
How is reverse phase chromatography different from regular chromatography?
Madhur L.
How is fractional distillation related to gas chromatography? How do you relate gas chromatography to what you know about thin layer chromatography? What is the mobile phase? What is the stationary phase? What are the differences in separation principles?
Study Questions • What is chromatography? • What is the origin of the name "chromatography"? • What is the difference between paper and thin-layer chromatography? • How are the mixtures separated during TLC chromatography? • What variables does the chromatographic separation depend on? • What is the difference between regular and two-dimensional chromatography? • What are advantages of two-dimensional chromatography? • Do you expect different inks to have the same components?
Adi S.
Recommended Textbooks
Biology for AP Courses
Objective Biology for NEET
Introduction to General, Organic and Biochemistry
Transcript
18,000,000+
Students on Numerade
Trusted by students at 8,000+ universities
Watch the video solution with this free unlock.
EMAIL
PASSWORD