The PCR reactions consist of primers, cell suspension; and PCR Master-Mix at a final volume of 50 pl: The Master-Mix contains PCR buffer; dNTPs, Taq polymerase and water: Each of the 50 ul reactions requires 1X PCR buffer; 0.2 mM dNTPs, and 1.25 Units of Taq polymerase_ Given the following stock concentrations: 10X PCR buffer; 10 mM dNTPs, and 5 Units/ul Taq polymerase, determine how you would prepare just enough of the Master-Mix for the six PCR reactions performed in your experiment: Water is used as the solvent in preparing the Master-Mix: Note: The final volume (50 pl) of each PCR reaction contains primers 4l) , extracted DNA (4 pl); and the master mix 2_ Why do we plug the black cable in at the top of the gel and the red cable at the bottom? What property of the DNA are we using during electrophoresis? 3_ Using _ the ''pseudomonad-specific"' primers, does obtaining band of approximately 1300 bp definitively establish your isolate O unknown is a pseudomonad? Does your negative control lane produce any bands (e.g , ~false positive" )? Are the bands produced by the isolates and unknowns the same size? What might account for the difference in size?