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Question 2 (25 Marks) a. Draw a graph of (log numbers of cells against time) of E. coli cells growing on a medium containing both glucose and lactose. Label to explain what is happening at each phase. [11 marks] b. What would determine the length of the lag phase of a culture? [4 marks] c. Describe how the electronic counter is used to determine the total numbers of cell in the population at stage 3. [2 marks] d. How would you determine how many of the cells in c. are dead? [5 marks] e. Give three ways in which you would confirm that your culture is really E. coli. [3 marks]

          Question 2 (25 Marks)
a. Draw a graph of (log numbers of cells against time) of E. coli cells growing on a medium containing both glucose and lactose. Label to explain what is happening at each phase.  [11 marks]
b. What would determine the length of the lag phase of a culture? [4 marks]
c. Describe how the electronic counter is used to determine the total numbers of cell in the population at stage 3. [2 marks]
d. How would you determine how many of the cells in c. are dead? [5 marks]
e. Give three ways in which you would confirm that your culture is really E. coli. [3 marks]

Question 2 (25 Marks)
a. Draw a graph of (log numbers of cells against time) of E. coli cells growing on a medium containing both glucose and lactose. Label to explain what is happening at each phase. [11 marks]
b. What would determine the length of the lag phase of a culture? [4 marks]
c. Describe how the electronic counter is used to determine the total numbers of cell in the population at stage 3. [2 marks]
d. How would you determine how many of the cells in c. are dead? [5 marks]
e. Give three ways in which you would confirm that your culture is really E. coli. [3 marks]

Added by Elizabeth F.

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