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Hello students, pyruvate kinase is an enzyme that catalyzes the conversion of phosphoenolpyruvate and adp to pyruvate and atp in glycolysis and plays a role in regulating cell metabolism.
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When this pyruvate kinase is deficient, it will not able to convert phosphoenolpyruvate to pyruvate and no production of atp.
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Also, all the upstream intermediates of glycolysis will get accumulated.
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One of that is 1 ,3 -bisphosphoglycerate.
00:35
Due to this deficiency of pyruvate kinase, there will be increase in accumulation of 1 ,3 -bisphosphoglycerate.
00:43
This will lead to synthesis of 2 ,3 -bisphosphoglycerate.
00:48
The function of this 2 ,3 -bisphosphoglycerate is that it act as a regulator of the allosteric properties of hemoglobin in the ibc.
00:58
Due to increase in the synthesis of this, it will result in lower the affinity of hemoglobin for oxygen and thus oxygen will release in tissue.
01:10
Second, next, hexokinase is an enzyme that catalyzes the atp -dependent phosphorylation of glucose to glucose 6 -phosphate.
01:20
This is the first step and the first rate limiting step in the glycolytic pathway.
01:28
When there is deficiency in this hexokinase, it will not able to convert this glucose into glucose 6 -phosphate...