Results:
Submit your work at the start of the next lab period. You may consult with others, but you are responsible for turning in your own work.
From the analysis in Part C, produce a graph, calculate the protein concentration of your UNDILUTED protein extract, and write a figure caption.
Write a scientific abstract for this week's lab. See Appendix for guidelines.
Analysis Questions:
Answer these questions on a separate piece of paper (typed or legibly handwritten) and submit your work at the start of the next lab period. You may consult with others, but you are responsible for turning in your own work.
1. Review Figure 2 from this week's Introduction. Suppose the Abs595 reading of a 1:10 dilution of a protein extract was 0.26. Calculate the protein concentration of the UNDILUTED protein extract. Show your work.
2. You have Abs595 values for three dilutions of your protein extract. In theory, you could calculate the protein concentration using all three Abs595 values, but they would likely lead to different values. What dilution of protein extract did you choose to determine your protein concentration, and why did you choose this value? Why was it necessary to make dilutions of the protein extract?
3. Examine the consolidated class data of protein concentrations and amounts. Can you make any general conclusions? Explain your answer.