Question

Sample questions for Exam III 1) RNAi can work through deadenylation T/F 2) You want to perform a global RNAi screen to identify all of the host cell genes that normally promote microbial growth. So, you transfect human cells with siRNAs and then infect them with your microbe expressing the green fluorescent protein. You should look for: a) increased fluorescence b) decreased fluorescence 3) Interactions involving rRNA are a key part of ribosome function T/F 4) Snurps are primarily found in the cytoplasm T/F 5) TATA-binding protein (TBP) is required for initiation by A) RNA polymerases I, II, and III B) RNA polymerases I and III C) RNA polymerase II D) RNA polymerase III E) RNA polymerase I 6) Methylation of a CpG island in a promoter would a) inhibit gene expression b) activate gene expression c) have no effect 7) An enhancer ______ the gene it affects. A) must be upstream from B) can be very distant from C) cannot be within an intron of D) must be in the same orientation as E) must be downstream from 8) the -10 and -35 elements are spatially conserved between prokaryotes and eukaryotes T/F

          Sample questions for Exam III
1)
RNAi can work through deadenylation T/F
2)
You want to perform a global RNAi screen to identify all of the host cell genes that normally
promote microbial growth. So, you transfect human cells with siRNAs and then infect them
with your microbe expressing the green fluorescent protein. You should look for:
a)
increased fluorescence
b) decreased fluorescence
3)
Interactions involving rRNA are a key part of ribosome function
T/F
4)
Snurps are primarily found in the cytoplasm
T/F
5)
TATA-binding protein (TBP) is required for initiation by
A) RNA polymerases I, II, and III
B) RNA polymerases I and III
C) RNA polymerase II
D) RNA polymerase III
E) RNA polymerase I
6) Methylation of a CpG island in a promoter would
a) inhibit gene expression
b) activate gene expression
c) have no effect
7)
An enhancer ______ the gene it affects.
A) must be upstream from
B) can be very distant from
C) cannot be within an intron of
D) must be in the same orientation as
E) must be downstream from
8)
the -10 and -35 elements are spatially conserved between prokaryotes and eukaryotes T/F

Sample questions for Exam III
1)
RNAi can work through deadenylation T/F
2)
You want to perform a global RNAi screen to identify all of the host cell genes that normally
promote microbial growth. So, you transfect human cells with siRNAs and then infect them
with your microbe expressing the green fluorescent protein. You should look for:
a)
increased fluorescence
b) decreased fluorescence
3)
Interactions involving rRNA are a key part of ribosome function
T/F
4)
Snurps are primarily found in the cytoplasm
T/F
5)
TATA-binding protein (TBP) is required for initiation by
A) RNA polymerases I, II, and III
B) RNA polymerases I and III
C) RNA polymerase II
D) RNA polymerase III
E) RNA polymerase I
6) Methylation of a CpG island in a promoter would
a) inhibit gene expression
b) activate gene expression
c) have no effect
7)
An enhancer the gene it affects.
A) must be upstream from
B) can be very distant from
C) cannot be within an intron of
D) must be in the same orientation as
E) must be downstream from
8)
the -10 and -35 elements are spatially conserved between prokaryotes and eukaryotes T/F

Added by Felipe C.

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