Sp23 Final Exam practice_Key.pdf (7.58 MB) H H OH OH 14. (4 pts) Matching. Choose a term listed on the left (labeled A-E) that best matches the description on the right. One term per description, but terms can be used more than once. A. G-protein coupled receptors C Single transmembrane domain B. Phospholipase C E Activated by binding cAMP C. Receptor tyrosine kinase B Inositol trisphosphate production D. Calcium ions A Binds epinephrine E. Protein kinase A D Second messenger C Autophosphorylation b Diacylglycerol production A Several transmembrane domains 15. (3 pts) Multiple choice. Circle the one letter that corresponds to the best answer.
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Madhur L.
Suppose that a protein tyrosine kinase (E) catalyzes a reaction with the kinetic mechanism shown below, in which a phosphate group from ATP is transferred to a Tyr residue on the target protein. Target Protein ATP Target Protein-OPO32- ADP E E-ATP E-ATP/Target Protein <=> E-ADP/Target Protein-OPO32- E-ADP E The protein kinase activity can be assayed by phosphorylation of a small peptide substrate containing the consensus specificity sequence from the target protein that includes the Tyr residue. A. How would you describe the kinetic mechanism of this protein kinase? (Cleland terminology; be precise) B. On the axes below, sketch the double reciprocal plots (1/vo vs. 1/[ATP] and 1/vo vs. 1/[peptide]) you would obtain if you assayed the protein kinase with three different concentrations of the other substrate. Indicate on the plots the relative concentrations of the other substrate. Sketch lines for velocities measured at 3 peptide concentrations, labeling lowest to highest peptide concentrations. Sketch lines for velocities measured at 3 ATP concentrations, labeling lowest to highest ATP concentrations. C. What general term is used to describe the central complexes E(ATP)(target peptide) and E(ADP)(target peptide-OPO32-) in this type of kinetic mechanism? D. Write the general reaction by which a cell would remove the phosphate group from the target protein. What enzyme(s) catalyze this process?
Sri K.
1) What causes the membrane potential to return to the normal negative value during the repolarization phase of an action potential? Select one: a. Closing of voltage-gated K+ channels b. Opening of ligand-gated Na+ channels c. Closing of voltage-gated K+ channels d. Opening of voltage-gated Na+ channels e. Opening of voltage-gated K+ channels 2) Neuronal cells on your taste buds initiate an action potential when they detect specific flavor molecules. Cell surface proteins that detect flavor molecules and initiate an action potential could be: Select one: a. Extracellularly ligand-gated sodium channels b. Intracellularly ligand-gated sodium channels c. Aquaporin channels d. Mechanically gated channels e. Potassium leak channels 3) Translocation of most proteins into the ER requires all of the following EXCEPT a(n): Select one: a. Signal recognition particle b. Signal sequence c. SRP receptor d. Importin receptor e. Ribosome 4) Which of the following describes a transporter that requires ATP hydrolysis to move solute A and solute B out of the cell? Select one: a. A passive symport b. An active uniport c. An active antiport d. A passive antiport e. An active symport 5) A vesicle traveling down an axon on a microtubule towards a synapse is being pulled by which type of motor? Select one: a. Dynein b. Kinesin c. Myosin d. Filamin e. Actin 6) Which of the following proteins is likely to require vesicular transport to reach its functional destination in the cell? Select one: a. The Na/K pump b. Signal peptidase c. RNA polymerase d. Aconitase (catalyzes the first step in the citric acid cycle) 7) Based on their location in the cell, we can conclude that the tubulin protein monomers that form microtubules must be synthesized... Select one: a. In the nucleus b. On rough ER c. By free ribosomes in the cytosol d. By tubulin synthase e. On smooth ER
Adi S.
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