Specify whether each of the statements below is TRUE OR FALSE and EXPLAIN your reasoning . PLEASE NOTE: NO POINTS WILL BE AWARDED IF YOU DO NOT PROVIDE AN EXPLANATION FOR YOUR CHOICE. a. The effects of cyclic AMP vary depending on the cell type because different cells express different isoforms of protein kinase A (0.4 points) b. Binding of a ligand to a receptor tyrosine kinase activates the cytosolic catalytic domain of the receptor by inducing a conformational change across the membrane through a single transmembrane segment. (0.4 points) You work in a developmental biology lab that uses Xenopus laevis (the African clawed frog) as a model organism. You are working on a project investigating the activity of different cadherins in cells. You insert a transgene to express RFP-E-cadherin (red) in neural crest cells from Xenopus embryos and GFP-N-Cadherin in a second set of neural crest cells from Xenopus embryos. These cells do not normally express ANY cadherins. Explain the results you expect to see in the following cultures. a. Culture A: cells that express RFP-E-cadherin (0.3 points) b. Culture B: A mixture of cells expression RFP-E-cadherin and GFP-N-Cadherin-expressing cells (0.4 points) c. Culture C: cells that express RFP-E-cadherin which have been transfected with an siRNA to knock-down the gene that codes for fibronectin (0.4 points) d. Culture D: Cells that express GFP-N-cadherin grown in the presence of EGTA, a compound that depletes free Ca 2+ concentration from the culture medium (0.4 points)
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The effects of cyclic AMP vary depending on the cell type because different cells express different isoforms of protein kinase A. **True**. The effects of cyclic AMP (cAMP) do vary depending on the cell type because different cells express different isoforms of Show more…
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5. A company produces cylindrical pop cans with internal base radius r = 5 cm and height h = 15 cm. They wish to reduce r by 0.1 cm. In order to keep the volume of pop the same, we find, using differentials, that h should change by: (a)0.6; (b)0.7; (c)0.8; (d)0.9; (e)1. Ans:(a) 6.Let f(x, y) = { x^2/(x^2 + y^2), (x, y) != (0, 0) c, (x, y) = (0, 0) for some constant c. For f to be continuous at (0, 0), c must equal (a)0; (b)1; (c)2; (d)3; (e) there is no such value of c. Ans:(e)
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