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Standard Curve for Protein Standard 1.6 1.4 Plot Area 1.2 Absorbance 1 0.8 Series1 0.6 0.4 Linear (Series1) 0.2 0 0 0.2 0.4 0.6 0.8 1 1.2 Concentration [mg/ml BSA] 4. Below is Table 2 that will be used to calculate the concentration of your unknowns, using the standard curve. When determining your unknown concentrations, use only those absorbance values that fall within the linear range of the standard curve. If the absorbance values fall outside this range, do not use them to calculate concentration for that dilution—just leave the 2 boxes to the right, blank. Fill out the table to determine the concentration of the unknown protein. Show your work in the standard curve. Table 2. Estimation of protein unknowns (4 pts) Unknown # Dilution factor Absorbance (OD Value) Std curve reading (protein concentration) Mg/ml Actual [] of sample Mg/ml Average [] of sample Mg/ml 1 1 2.4 1 5 1.28 1 10 0.64 1 20 0.37

          Standard Curve for Protein Standard
1.6
1.4 Plot Area
1.2
Absorbance 1
0.8
Series1
0.6
0.4
Linear (Series1)
0.2
0
0 0.2 0.4 0.6 0.8 1 1.2
Concentration [mg/ml BSA]
4. Below is Table 2 that will be used to calculate the concentration of your unknowns, using the standard curve. When determining your unknown concentrations, use only those absorbance values that fall within the linear range of the standard curve. If the absorbance values fall outside this range, do not use them to calculate concentration for that dilution—just leave the 2 boxes to the right, blank. Fill out the table to determine the concentration of the unknown protein. Show your work in the standard curve.
Table 2. Estimation of protein unknowns (4 pts)
Unknown # Dilution factor Absorbance (OD Value) Std curve reading (protein concentration) Mg/ml Actual [] of sample Mg/ml Average [] of sample Mg/ml
1 1 2.4
1 5 1.28
1 10 0.64
1 20 0.37

Standard Curve for Protein Standard
1.6
1.4 Plot Area
1.2
Absorbance 1
0.8
Series1
0.6
0.4
Linear (Series1)
0.2
0
0 0.2 0.4 0.6 0.8 1 1.2
Concentration [mg/ml BSA]
4. Below is Table 2 that will be used to calculate the concentration of your unknowns, using the standard curve. When determining your unknown concentrations, use only those absorbance values that fall within the linear range of the standard curve. If the absorbance values fall outside this range, do not use them to calculate concentration for that dilution—just leave the 2 boxes to the right, blank. Fill out the table to determine the concentration of the unknown protein. Show your work in the standard curve.
Table 2. Estimation of protein unknowns (4 pts)
Unknown # Dilution factor Absorbance (OD Value) Std curve reading (protein concentration) Mg/ml Actual [] of sample Mg/ml Average [] of sample Mg/ml
1 1 2.4
1 5 1.28
1 10 0.64
1 20 0.37

Added by Emily M.

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