00:01
Basically is to organize the steps of gene transfer method for gene therapy using the viral vector approach.
00:10
So let's say the very first step for this is the gene of interest is cloned into the retrovirus.
00:19
This is step number one.
00:37
So this is the gene of interest first.
00:41
The next step, once you have the gene in interest, you infect, you transfect the host cell with the retrovirus.
00:51
So the coat of the retrovirus is disassembled.
01:15
And the next step is that, so once the the membrane or the coat of the retrovirus is disassembled and then the the genome or the gene of the retrovirus that actually enters cell by cell membrane fusion.
01:34
So this one is the viral rna that has the gene of interest actually release, i would say enter the cell by membrane fusion.
02:24
So this tells you how the viral rna enters the cell.
02:28
So once that enters the cell, the viral dna continuity of interest will release into the cell of plasm...