Question

Sterilization can be achieved by putting an item in the autoclave for 15 minutes at 121Â°C. To achieve sterilization of the same item in an oven with dry heat, name two things that would need to be changed. The item is reusable and capable of withstanding high heat. A vial containing spores from the bacterium Bacillus stearothermophilus was used to test our autoclave this month. After running one regular autoclave cycle and placing the vial in an incubator for 24 hours, the vial turned a yellow color. Is the autoclave running properly, or should you call to get it repaired? Name one reason supporting your answer. Susceptibility to Bacitracin allows you to differentiate between which groups of streptococci? What groups of bacteria are differentiated using Optochin?

          Sterilization can be achieved by putting an item in the autoclave for 15 minutes at 121Â°C. To achieve sterilization of the same item in an oven with dry heat, name two things that would need to be changed. The item is reusable and capable of withstanding high heat.
A vial containing spores from the bacterium Bacillus stearothermophilus was used to test our autoclave this month. After running one regular autoclave cycle and placing the vial in an incubator for 24 hours, the vial turned a yellow color.
Is the autoclave running properly, or should you call to get it repaired? Name one reason supporting your answer.
Susceptibility to Bacitracin allows you to differentiate between which groups of streptococci?
What groups of bacteria are differentiated using Optochin?

Added by Heather B.

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