Question

The marker comes in two alleles or versions. In a PCR reaction with primers flanking the region of interest, the first allele can produce a DNA fragment of approximately 100bp, while the second allele produces a DNA fragment of approximately 300bp. You carry out the PCR of the five DNA samples and visualize the results by gel electrophoresis as shown in figure 1. Muestra de la escena del crimen Sospechosos Std. S1 S2 S3 S4 600 bp 500 bp 400 bp 300 bp 200 bp 100 bp Figure 1. Results of the PCR products on an agarose gel. Answer the following questions: 1. Which of the suspects' DNA matches the crime scene DNA? Based on the results, is this the person who committed the crime?

          The marker comes in two alleles or versions. In a PCR reaction with primers flanking the region of interest, the first allele can produce a DNA fragment of approximately 100bp, while the second allele produces a DNA fragment of approximately 300bp. You carry out the PCR of the five DNA samples and visualize the results by gel electrophoresis as shown in figure 1.

Muestra de la escena del crimen
Sospechosos
Std. S1 S2 S3 S4
600 bp
500 bp
400 bp
300 bp
200 bp
100 bp

Figure 1. Results of the PCR products on an agarose gel.
Answer the following questions:
1. Which of the suspects' DNA matches the crime scene DNA? Based on the results, is this the person who committed the crime?

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The marker comes in two alleles or versions. In a PCR reaction with primers flanking the region of interest, the first allele can produce a DNA fragment of approximately 100bp, while the second allele produces a DNA fragment of approximately 300bp. You carry out the PCR of the five DNA samples and visualize the results by gel electrophoresis as shown in figure 1.

Muestra de la escena del crimen
Sospechosos
Std. S1 S2 S3 S4
600 bp
500 bp
400 bp
300 bp
200 bp
100 bp

Figure 1. Results of the PCR products on an agarose gel.
Answer the following questions:
1. Which of the suspects' DNA matches the crime scene DNA? Based on the results, is this the person who committed the crime?

Added by Joan M.

Biology for AP Courses

Julianne Zedalis, John Eggebrecht

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Solved by Expert Adi S

06/29/2023

Step 1

To begin, the forensic PCR technique is used to examine a particular genetic marker on a hair left at the crime scene. The marker comes in two alleles or versions, and in a PCR reaction with primers flanking the region of interest, the first allele can produce a Show more…

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Suppose you are working in a forensic laboratory. You have just received a DNA sample from a hair left at the crime scene, along with DNA samples from (4) possible suspects. Your job is to examine a particular genetic marker and see if any of the three suspects match the hair DNA for this marker. The marker comes in two alleles or versions. In a PCR reaction with primers flanking the region of interest, the first allele can produce a DNA fragment of approximately 100bp, while the second allele produces a DNA fragment of approximately 300bp. You carry out the PCR of the five DNA samples and visualize the results by gel electrophoresis as shown in figure 1. Answer the following questions: 1. Which of the suspects' DNA matches the crime scene DNA? Based on the results, is this the person who committed the crime? 2. Where could we apply this forensic PCR technique apart from solving a crime such as murder?

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