Suppose you transform competent cells with a plasmid. You spread 50 µl of your transformation mix on a CSM-Leu plate and incubate it overnight. You save the remaining transformation mix in the refrigerator. The next day, the plate has 400 colonies. Now you want to spread the remaining transformation mix on fresh plates so that you will get 200 colonies per plate. What volume (µl) should you spread on each plate?
Added by Jamie B.
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