Complete the following table. Which donor can give blood safely without causing an agglutination reaction? Your answer will depend on which antibodies the recipient has. Recipient's Blood Type RBC Antigen Plasma Antibodies Donor O Donor A Donor B Donor AB Type A Anti-B A antigen antibodies Type B Anti-A B antigen antibodies Type AB No antibodies No antigens O Negative Anti-A and Anti-B No antigens antibodies
Blood Typing Procedures:
Procedure:
1. Label each blood typing slide: Slide 1: Mr. Smith / Slide 2: Mr. Jones / Slide 3: Mr. Green / Slide 4: Ms. Brown
2. Place three to four drops of Mr. Smith's blood in each of the A, B, and Rh wells of Slide 1
3. Place three to four drops of Mr. Jones's blood in each of the A, B, and Rh wells of Slide 2
4. Place three to four drops of Mr. Green's blood in each of the A, B, and Rh wells of Slide 3.
5. Place three to four drops of Ms. Brown's blood in each of the A, B, and Rh wells of Slide 4.
6. Place three to four drops of simulated anti-A serum in each A well on the four slides.
7. Place three to four drops of simulated anti-B serum in each B well on the four slides.
8. Place three to four drops of simulated anti-Rh serum in each Rh well on the four slides.
9. Obtain three toothpicks per blood typing slide. Stir each well with a separate clean toothpick.
10. Observe each slide and record your observations in the following table.
11. Clean up. Simulated Blood is non-biological and nontoxic and may be flushed down the drain.
Results and Analysis:
Anti-A Serum
Anti-B Serum
Anti-Rh Serum
Blood Type
Slide 1: Mr. Smith
Slide 2: Mr. Jones
Slide 3: Mr. Green
Slide 4: Ms. Brown
Check Your Understanding:
1. Antigens in the blood are located ______ while antibodies are located ______
2. What is the adverse reaction that occurs in incompatible (mismatched) blood transfusion?