Tetracycline antibiotic was given to a 30 year old client with Chlamydia infection. What is the mechanism of action of the drug? Question 4 options: It prevents the replication of bacteria. It alters the configuration of bacterial cytoplasm. It interferes with the function of bacterial ribosomes. It inhibits the functions of bacterial mitochondria.
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Tetracycline is an antibiotic used to treat bacterial infections in humans because it prevents the growth and spread of bacteria. This drug is known to bind strongly and specifically to the A site in the 30S ribosomal subunit. Based on this information you would expect that: a. tetracycline does not affect translation in eukaryotic cells. b. tetracycline inhibits RNA splicing in prokaryotes. c. tetracycline will prevent the small ribosomal subunit from binding to the Shine-Dalgarno sequence. d. tetracycline prevents the growth of bacteria by blocking the binding of charged-tRNA molecules to the complex of the small ribosomal subunit + mRNA. e. tetracycline prevents the fMet-tRNA's anticodon from base-pairing with the AUG codon.
Madhur L.
Many different antibiotics affect steps in translation. Based on the mode of action of each antibiotic, suggest a possible mechanism by which it works. a. Chloramphenicol blocks the peptidyl transferase activity. b. Tetracycline blocks the A site. c. Streptomycin binds to the 30S subunit and results in misreading the mRNA.
QUESTIONS: 1. Which two antibiotics were the most effective overall? What are their mechanisms of action? Amoxicillin as well as Tetracycline were most effective overall. Tetracycline's mechanism of action is that it ultimately inhibits protein synthesis, leading to a bacteriostatic effect. Amoxicillin's mechanism of action is unknown. 2. Which two were the least effective overall? What are their mechanisms of action? 3. Why would one antibiotic be effective against a Gram-positive cell but not a Gram-negative? Give an example from the results. 4. Would the spread plate technique or pour plate be best to seed the lawn of bacteria? Why?
Dominador T.
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