A ab Aa ... 1. What is the complementary DNA sequence? DNA TAC GGA AAG ACA AGC GCA ATA AAT GGG CTG TGG TTC AAT GGG CTC ACC CTG DNA ATG CCT TTC TGT TCG CGT TAT TTA CCC CCC GAC AC ACC AAG TTA CCC GAG TGG GAC 2. Transcribe and translate the sequence from above: DNA TAC GGA AAG ACA AGC GCA ATA AAT GGG CTG TGG TTC AAT GGG CTC ACC CTG RNA AUG CCU UUC UGU UCG CGU UAU UUA CCC GAC ACC AAG UUA CCC GAG UGG GAC AA 3. What if there was a deletion of the fourth nucleotide? It will disrupt the rest of codes. Transcribe and translate that sequence below. DNA TAC GAA AGA CAA GCG CAA TAA ATG GGC TGT GGT TCA ATG GGC TCA CCC TG RNA AUG CUU UCU GUU CGC GUU AUU UAC CCG ACA CCA AGU UAC CCG AGU GGG AC AA Does the resulting amino acid sequence look the same as in question 2? No Why not? 4. What if there was a substitution in the eighth codon, and the DNA sequence now read ATT instead of AAT? DNA TAC GGA AAG ACA AGC GCA ATA ATT GGG CTG TGG TTC AAT GGG CTC ACC CTG RNA AUG CCU UUC UGU UCG CGU UAU UAA CCC GAC ACC AAG UUA CCC GAG UGG GAC AA
Added by Kimberly W.
Close
Step 1
The complementary bases are as follows: - Adenine (A) pairs with Thymine (T) - Thymine (T) pairs with Adenine (A) - Guanine (G) pairs with Cytosine (C) - Cytosine (C) pairs with Guanine (G) Given the DNA sequence TAC GGA, the complementary DNA sequence would be Show moreā¦
Show all steps
Your feedback will help us improve your experience
Jiva Y and 85 other Biology educators are ready to help you.
Ask a new question
Labs
Want to see this concept in action?
Explore this concept interactively to see how it behaves as you change inputs.
Key Concepts
Recommended Videos
Here is the DNA sequence of part of the template strand for wildtype TP53 gene (The entire sequence is 1179 nucleotides long, this is just a small portion of it): 3'- TCA AGG ACG TAC CCG CCG TAC TTG GCC TCC GGG TAG GAG TGG TAG TAG TGT GAC -5' 1A) Write the mRNA sequence for wildtype TP53. 1B) Use the codon table (provided below) to determine the sequence of amino acids in the resulting p53 protein fragment. (use the 3-letter abbreviations for each amino acid, e.g. Proline = Pro) Here is the DNA sequence of the same part of the template strand for mutant TP53 gene: 3'- TCA AGG ACG TAC CCG CCG TAC TTG GTC TCC GGG TAG GAG TGG TAG TAG TGT GAC -5' 2A) Write the mRNA sequence for mutant TP53. 2B) Use the codon table to determine the sequence of amino acids in the resulting p53 protein fragment.
Jiva Y.
a) Transcribe and translate the following DNA. Label any nucleic acids with their 5' and 3' ends. b) Using the DNA sequence above, write a new DNA sequence from 3' to 5' that incorporates a silent mutation in the third amino acid. Bold or underline the nucleotide that has been changed. c) Using the DNA sequence above, write a new DNA sequence from 3' to 5' that incorporates a nonsense mutation. DO NOT change the original Stop codon. Bold or underline the nucleotide that has been changed. d) Using the DNA sequence above, write a new DNA sequence from 3' to 5' that incorporates a missense mutation in the second amino acid. Bold or underline the nucleotide that has been changed. I. What is the correct order of enzyme action during DNA replication? Number the steps from 1 to 7. 1. Binding to origin of replication (AT rich sequence) 2. Helicase/topoisomerase activity 3. Single-stranded binding proteins bind and prevent reannealing 4. Synthesis of RNA primers (priming) 5. DNA polymerization/proofreading 6. 5'-3' exonuclease activity/DNA polymerization 7. Ligation II. Using the genetic code table below, please answer the following questions: SECOND LETTER - A T ucul UAU Tyr UGU Cy U uuc uce UACS uac) UUA UCA UGA A uua} UCG) UAG Amber ter UGG Trp G cw) ccu CAU). His CaU) cuc ccc CAC Leu CGC CUA CCA CAA CGA cual cca CAG Gin CGa FIRST 5LETTER THIRD 3LETTER (nnv ACU UAU) AGU) U AUcne ACC AAC aGc) AUA) ACA AAA AGA) AUG Met ACG) AAG GG Gu) (ooo GAU)ASP GGU] GUC acc GAC GGC Val DU4 GUA GCA GAA) GGA Glu a) Transcribe and translate the following DNA. Label any nucleic acids with their 5' and 3' ends. DNA3 TACCCAACAATC 5' b) Write a new DNA sequence from 3' to 5' that incorporates a silent mutation in the third amino acid. Bold or underline the nucleotide that has been changed. c) Write a new DNA sequence from 3' to 5' that incorporates a nonsense mutation. DO NOT change the original Stop codon. Bold or underline the nucleotide that has been changed. d) Write a new DNA sequence from 3' to 5' that incorporates a missense mutation in the second amino acid. Bold or underline the nucleotide that has been changed.
Madhur L.
Now imagine that RNA polymerase is transcribing the following sequence of DNA. The underlined portions represent the nucleic acids encoding protein domains important for enzymatic catalysis. The transcribed gene has two exons and one intron (intron is in lowercase and in italics). The initial start codon is bolded. Define protein domains and active site. 5'-AGG TCT AGG CAC TCG TCG ATG ATC TAT CTA CGC GTG TTT CGG TTa gaa gta tgg atg aga tac tcc tgg ata gcg gga taC GAT TGG ACA GGC ACC GAT GAC GGG CCG CAA CAG AAT CGA CCC ATA TGA AAA GC-3' Give the amino acid sequence for the protein that is encoded by this segment of mRNA. Write your answer using the one-letter amino acid code. Changes to the DNA sequence often only involve only one nucleotide. Shown below are four different DNA mutations that each contains a different nucleotide change from the original sequence. For each mutant allele, specifically describe the nature (synonymous, missense, nonsense, frameshift), location of the mutation and the effect on the primary sequence of the protein. Sequence for mutant allele 1 is 5'-AGG TCT AGG CAC TCG TCG ATG ATC TAT CTA GGC GTG TTT CGG TTa gaa gta tgg atg aga tac tcc tgg ata gcg gga taC GAT TGG ACA GGC ACC GAT GAC GGG CCG CAA CAG AAT CGA CCC ATA TGA AAA GC-3' Sequence for mutant allele 2 is 5'-AGG TCT AGG CAC TCG TCG ATG ATC TAT CTA CGC GTG TTT CGG TTa gaa gta tgg atg aga tac tcc tgg ata gcg gga taC GAT TGG ACA GGC ACC GAC TGA CGG GCC GCA ACA GAA TCG ACC CAT ATG AAA AGC-3' Sequence for mutant allele 3 is 5'-AGG TCT AGG CAC TCT TCG ATG ATC TAT CTA CGC GTG TTT CGG TTa gaa gta tgg atg aga tac tcc tgg ata gcg gga taC GAT TGG ACA GGC ACC GAT GAC GGG CCG CAA CAG AAT CGA CCC ATA TGA AAA GC-3'
Maitreya E.
Recommended Textbooks
Biology for AP Courses
Objective Biology for NEET
Introduction to General, Organic and Biochemistry
Transcript
18,000,000+
Students on Numerade
Trusted by students at 8,000+ universities
Watch the video solution with this free unlock.
EMAIL
PASSWORD