Texts: HAND TRANSMISSION OF MICROORGANISMS (Purpose) Method: Microflora, Micrococcus Questions: 1. Was the number of colonies that formed affected by the student's position in the handshake line? 2. Did increasing the number of washes significantly change the number of colonies that grew? 3. Determine which method of handwashing was most effective. List recommendations for how someone should clean their hands (include the method and the number of washes if necessary, one and two washes).
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We can do this by dividing the students into different groups based on their position (e.g., front, middle, back) and comparing the number of colonies that formed in each group. Show more…
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What method was used to determine the microbe population on hands? a. Fingerprints on a TSA plate b. Plate counts enumeration of the rinse water c. Gram staining swab samples d. DNA gene sequencing of the bacteria from palms e. Negative staining all the colonies What best describes the number of different bacteria the microbiologists found on the test subjects hands? a. Relatively few bacteria were found on the hands. b. Most of the hands had deadly pathogenic bacteria. c. Women's hands had a greater diversity of bacterial species than men's hands d. Most hands had the same kinds of bacterial species e. No hands had E. colicontamination
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REVIEW QUESTIONS 1. Based on your results, which disinfectant(s) worked best against the bacteria you tested? Explain. Explain why hand soap (Triclosan) worked best to disinfect against S. Aureus Explain why Mr. Clean (sodium hydroxide) worked best to disinfect against E. Coli 2. Based on your results, which disinfectant(s) were least effective? Explain. Explain why Mr. Clean (sodium hydroxide) was ineffective to disinfect against S. Aureus Explain why mouthwash (Cetylpyridinium chloride) was ineffective to disinfect against E. Coli 3. Compare your plate with that of other group who tested different bacterium. Did you see the same pattern of zones of inhibition for both bacteria tested? Explain. S. Aureus E. coli
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