the 3' to 5' direction so it can bind to mRNA running in the 5' to 3' direction. So, the anticodon of this tRNA is at the cloverleaf. Determine which amino acid this tRNA is charged. Enter its three-letter box in the image. You can view it by consulting your textbook or click Show the mRNA codon table. Note: You will need to close the input your answer. Today, protein engineers create unnatural proteins that incorporate new amino acids. To start, tRNAs by mutating the anticodon-encoding segment of a tRNA gene, like the one in this problem popular unnatural tRNAs contains the anticodon 3'-AUU-5'. While different organisms produce very few living species naturally produce tRNA with this anticodon, because this tRNA would never base-pair with mRNA contain an unnatural nucleotide base pair with a stop codon. Protein engineers then mutate a natural aminoacyl-tRNA synthetase such that it binds this unnatural it with an unnatural amino acid. Which of the following domains in aminoacyl-tRNA synthetase the enzyme to be able to charge an unnatural tRNA with an unnatural amino acid? Check all that Anticodon binding site ATP binding site Amino acid binding site