Question

6. The agarose gel at the right shows the pattern of bands obtained when a particular fragment of double-stranded DNA is digested to completion with the restriction enzymes shown. a. Which of the six restriction maps shown below is consistent with the observed pattern of restriction fragments? b. The DNA from this gel was then blotted onto a nylon filter. The filter was probed at high stringency with a radioactively labeled DNA sequence from the gene pep, which is known to lie somewhere within the region of DNA being studied here. The image to the right shows the pattern of radioactivity observed on the autoradiograph. Where is the pep gene located in the correct restriction map?

          6. The agarose gel at the right shows the pattern of bands obtained when a particular fragment of double-stranded DNA is digested to completion with the restriction enzymes shown.

a. Which of the six restriction maps shown below is consistent with the observed pattern of restriction fragments?

b. The DNA from this gel was then blotted onto a nylon filter. The filter was probed at high stringency with a radioactively labeled DNA sequence from the gene pep, which is known to lie somewhere within the region of DNA being studied here. The image to the right shows the pattern of radioactivity observed on the autoradiograph.

Where is the pep gene located in the correct restriction map?

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6. The agarose gel at the right shows the pattern of bands obtained when a particular fragment of double-stranded DNA is digested to completion with the restriction enzymes shown.

a. Which of the six restriction maps shown below is consistent with the observed pattern of restriction fragments?

b. The DNA from this gel was then blotted onto a nylon filter. The filter was probed at high stringency with a radioactively labeled DNA sequence from the gene pep, which is known to lie somewhere within the region of DNA being studied here. The image to the right shows the pattern of radioactivity observed on the autoradiograph.

Where is the pep gene located in the correct restriction map?

Added by Kayla B.

Biology for AP Courses

Julianne Zedalis, John Eggebrecht

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Solved by Expert Farhan Anwar

09/12/2022

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Note the bands for E (EcoRI), N (NcoI), A (AvaIII), and combinations. Show more…

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The agarose gel at the right shows the pattern of bands obtained when a particular fragment of double-stranded DNA is digested to completion with the restriction enzymes shown. a. Which of the six restriction maps shown below is consistent with the observed pattern of restriction fragments? b. The DNA from this gel was then blotted onto a nylon filter. The filter was probed at high stringency with a radioactively labeled DNA sequence from the gene pep, which is known to lie somewhere within the region of DNA being studied here. The image to the right shows the pattern of radioactivity observed on the autoradiograph. Where is the pep gene located in the correct restriction map?

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Transcript

00:02 Okay, so, uh, looks like part b doesn't actually have a question, so i'm not really sure what the question would be there.

00:07 Oh, wait, no, it does have a question.

00:09 It's being blocked.

00:10 All right.

00:11 Let's just go through a first.

00:14 So, uh, the first question is, the augurus gel at the right shows the pattern of bands obtained when a particular fragment of double strand dnas digested to completion with restriction enzymes shown, which of the six restriction maps are shown below.

00:28 I've already drawn out the correct map, so let's go through this.

00:30 And i have the different fragments.

00:32 So a, first things first, a is 9kb and 3kb.

00:40 I think i have this drawn correctly.

01:05 Oh, oops, i actually definitely drew the wrong one.

01:07 All right, that's no matter, actually.

01:10 I'll reuse the map.

01:12 Okay, so the correct one is, let's see one.

01:16 Whoops, 2, 4.

01:24 All right, so the correct map is this.

01:29 N is at 3 kb.

01:33 A is at 4kb.

01:36 E is at 6kb.

01:40 And then e is at 11kb.

01:46 Okay, so why is this? so let's go through the different digest products.

01:50 All right, so a, if you cut with a, we have 3kb and 9kb.

01:54 So here, that's n...

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