00:02
Okay, so the question is referring to a figure that was not included, but we can kind of make to without it.
00:08
So essentially, we have a augurus gel, what this is right here.
00:17
And in our gel, we have various wells, loaded with dna.
00:26
I'm assuming from wild type, which i'm showing in blue, and the two mutants in purple and green.
00:31
I'm just put in the middle right here.
00:32
And they're saying that they have traveled a particular distance, and that the question is, where would these bands fall within this augurus gel? the basic premise, however, just a review of the augurus gel, is that the gel is made up of auguros, which when liquid is, when warm, is when hot, when melted, it is liquid.
01:01
When it solidifies, it creates kind of a matrix.
01:04
Mesh and so smaller dna pieces will tend to travel through this mesh easier than say larger pieces or fragments of dna and so we can differentiate by size now if i understand the question correctly the initial size of the gene of the wild type gene aa is not was not included but let's just assume that this is uh oops not 1 kb because that's not going to work.
01:44
Let's say 2kb.
01:45
So it's 2kb is the normal size of this wild type gene.
01:51
Now, let's say the a1 mutant has a 2 kilobase pair insert of dna into the wild type fragment, meaning that 2kb was inserted in the center, or rather not in the center, but into the gene itself...