The Bradford assay only works at low protein concentrations (from 0.05 to 0.5 mg/mL protein). How can we reduce the concentration of protein in our sample? a) Evaporate the sample b) Purify the sample c) Concentrate the sample d) Dilute the sample
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Step 1: Dilute the protein sample by adding a suitable amount of buffer or solvent to reduce the concentration of protein in the sample. Show more…
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If your calculated protein concentration is 0.43 mg/mL and that was calculated for a sample that was a 50-fold dilution, what is the concentration (in mg/mL) of your protein? In the Bradford assay, you need to add 100 ̀μ́L of a "protein solution" to your 5 mL of 1X Bradford reagent to make a sample. If you add 25 ̀μ́L of a 2 mg/mL stock protein and 75 ̀μ́L of buffer as your "protein solution", what is the concentration of protein in your "protein solution"
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