The diagram below shows the 'lac operon' which is a control system of gene expression regulating lactose metabolism in the bacterium E. coli. Genes z, y and a control the synthesis of the enzymes which are responsible for the metabolism of lactose in the bacterial cell. Gene i is a regulator gene controlling the expression of genes z, y and a. p is the promotor region to which RNA polymerase attaches during transcription of DNA to mRNA. o is the operator site to which the repressor protein can attach.
In the presence of lactose, the bacteria can produce the enzymes to metabolize lactose. If the bacteria are subculture onto a lactose free substrate, they cease to produce the enzymes for metabolizing lactose, within three minutes.
(a) Describe how the lac operon system works to repress the formation of the lactose metabolizing enzymes.