Question

4. The enzyme Fumarase catalyzes the following reaction: S + E ? ES ? P + E where k1 and k-1 are the rate constants of the reversible reaction step (forward and reverse reaction, respectively) and k2 is for the irreversible step. The values of k1, k-1, and k2 are 10^9 M^-1s^-1, 4.4 x 10^4 s^-1, and 10^3 s^-1. (a) What is the value of the Michaelis constant for fumarase? (b) At an enzyme concentration of 10^-6 M, what will be the initial rate of product formation at an initial substrate concentration of 10^-3 M?

          4. The enzyme Fumarase catalyzes the following reaction:
S + E ? ES ? P + E
where k1 and k-1 are the rate constants of the reversible reaction step (forward and reverse reaction, respectively) and k2 is for the irreversible step. The values of k1, k-1, and k2 are
10^9 M^-1s^-1, 4.4 x 10^4 s^-1, and 10^3 s^-1.
(a) What is the value of the Michaelis constant for fumarase?
(b) At an enzyme concentration of 10^-6 M, what will be the initial rate of product formation at an initial substrate concentration of 10^-3 M?

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4. The enzyme Fumarase catalyzes the following reaction:
S + E ? ES ? P + E
where k1 and k-1 are the rate constants of the reversible reaction step (forward and reverse reaction, respectively) and k2 is for the irreversible step. The values of k1, k-1, and k2 are
10^9 M^-1s^-1, 4.4 x 10^4 s^-1, and 10^3 s^-1.
(a) What is the value of the Michaelis constant for fumarase?
(b) At an enzyme concentration of 10^-6 M, what will be the initial rate of product formation at an initial substrate concentration of 10^-3 M?

Added by Kristen B.

Biology for AP Courses

Julianne Zedalis, John Eggebrecht

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Solved by Expert Madhur L

10/17/2022

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The reaction catalyzed by Fumarase is: \[ S + E \leftrightarrow ES \rightarrow P + E \] where: - \( k_1 = 10^9 \, \text{M}^{-1}\text{s}^{-1} \) (rate constant for the forward reaction) - \( k_{-1} = 4.4 \times 10^4 \, \text{s}^{-1} \) (rate constant for the Show more…

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The enzyme Fumarase catalyzes the following reaction: S + E ↔ ES → P + E where k1 and k-1 are the rate constants of the reversible reaction step (forward and reverse reaction, respectively) and k2 is for the irreversible step. The values of k1, k-1, and k2 are 10^9 M^-1s^-1, 4.4 x 10^4 s^-1, and 10^3 s^-1, respectively. (a) What is the value of the Michaelis constant for fumarase? (b) At an enzyme concentration of 10^-6 M, what will be the initial rate of product formation at an initial substrate concentration of 10^-3 M?

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