Question

The enzyme lactate dehydrogenase catalyses the reaction shown below. Incubation of 4R-2H-NADH with enzyme and pyruvate gives no incorporation of deuterium in the lactate product. However, incubation of 4S-2H-NADH with enzyme and pyruvate gives of 2S-2H-lactate. Write a mechanism for this reaction to explain these results.

Name: The enzyme lactate dehydrogenase catalyses the reaction shown below. Incubation of 4R-2H-NADH with enzyme and pyruvate gives no incorporation of deuterium in the lactate product. However, incubation of 4S-2H-NADH with enzyme and pyruvate gives of 2S-2H-lactate. Write a mechanism for this reaction to explain these results.
Uploaded: 2023-02-17T14:52:25-08:00
Duration: 2 min 33 s
Channel: Madhur L
Description: The enzyme lactate dehydrogenase catalyses the reaction shown below. Incubation of 4R-2H-NADH with enzyme and pyruvate gives no incorporation of deuterium in the lactate product. However, incubation of 4S-2H-NADH with enzyme and pyruvate gives of 2S-2H-lactate. Write a mechanism for this reaction to explain these results.

          The enzyme lactate dehydrogenase catalyses the reaction shown below. Incubation of 4R-2H-NADH with enzyme and pyruvate gives no incorporation of deuterium in the lactate product. However, incubation of 4S-2H-NADH with enzyme and pyruvate gives of 2S-2H-lactate. Write a mechanism for this reaction to explain these results.

The enzyme lactate dehydrogenase catalyses the reaction shown below. Incubation of 4R-2H-NADH with enzyme and pyruvate gives no incorporation of deuterium in the lactate product. However, incubation of 4S-2H-NADH with enzyme and pyruvate gives of 2S-2H-lactate. Write a mechanism for this reaction to explain these results.

Added by Jerry P.

Chemistry: Structure and Properties

Nivaldo Tro 2nd Edition

Instant Answer

Solved by Expert Madhur L

02/17/2023

Step 1

The enzyme converts pyruvate to lactate using NADH as a cofactor, where NADH is oxidized to NAD+. Show more…

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The enzyme lactate dehydrogenase catalyzes the reaction shown below. Incubation of 4R-2H-NADH with enzyme and pyruvate gives no incorporation of deuterium in the lactate product. However, incubation of 4S-2H-NADH with enzyme and pyruvate gives 2S-2H-lactate. Write a mechanism for this reaction to explain these results.