The enzyme trypsin is a protease that splits peptide bonds on the carboxyl side of lysine or arginine. Treatment of a hexapeptide produces arg + ser-ala + leu-gly-lys. Which of the following are possible sequences of the amino acids in the hexapeptide? (There is at least one correct answer, maybe more.) arg-leu-gly-lys-ser-ala leu-gly-lys-arg-ser-ala ser-ala-leu-gly-lys-arg leu-gly-lys-ser-ala-arg ser-ala-arg-leu-gly-lys arg-ser-ala-leu-gly-lys
Added by Gary J.
Close
Step 1
** Show more…
Show all steps
Your feedback will help us improve your experience
Farhan Anwar and 96 other Biology educators are ready to help you.
Ask a new question
Labs
Want to see this concept in action?
Explore this concept interactively to see how it behaves as you change inputs.
Key Concepts
Recommended Videos
You have to determine the amino acid sequence of a peptide. You performed the following steps using the enzyme cleavage of your peptide combined with the subsequent sequencing: Step 1. Treatment with trypsin yielded three fragments with the following sequences: Tyr – Leu – Asp – Arg; Gly – Ser – Ala – Lys; Trp – Gly – Ser – Met. Step 2. Treatment with pepsin gave the same three peptide fragments: Tyr – Leu – Asp – Arg; Gly – Ser – Ala – Lys; Trp – Gly – Ser – Met.
Madhur L.
Another endoprotease is trypsin. Trypsin hydrolyzes peptide bonds on the carboxyl side of lysine and arginine. If the following peptide sequence is hydrolyzed by trypsin, how many fragments will there be? Use the three-letter amino acid abbreviations to write the fragments out. Ala-Phe-Lys-Cys-Gly-Asp-Arg-Leu-Leu-Phe-Gly-Ala
Which amide bonds in the following polypeptide are cleaved by trypsin? By chymotrypsin? $$Phe-Leu-Met-Lys-Tyr-Asp-Gly-Gly-Arg-Val-Ile-Pro-Tyr$$
Recommended Textbooks
Biology for AP Courses
Objective Biology for NEET
Introduction to General, Organic and Biochemistry
Transcript
Watch the video solution with this free unlock.
EMAIL
PASSWORD