00:01
Hello students, the question is the following are not needed in the pcr reaction.
00:08
We have to explain why each component and how is what these enzymes do accomplished in the pcr reaction or pcr technique.
00:23
We have given helicase and primase.
00:31
Pcr if we see it is a laboratory technique used to amplify a specific dna sequence.
00:41
It amplifies a specific dna sequence and it involves three steps, three main steps that is denaturation, second one is annealing and third if we see extension.
01:20
During denaturation the double -stranded dna template is separated into two single strands and during annealing the short dna primer it bind to the complementary sequences on each strand and during extension what happens is a dna polymerase enzyme synthesize a dna sequence and the dna complementary to the template strand.
01:47
So if we see helicase, helicase is an enzyme that unwinds the, it unwinds the double -stranded dna during replication.
02:20
If we see the in pcr the dna template is already denatured by heating.
02:27
So helicase is not needed.
02:30
So we'll not need helicase...