The following electropherogram shows that the peak labeled as 20.3 in the D12S391 locus and the two OLs in the SE33 locus were not true DNA alleles. What are these peaks? What is causing them? (4 points)
Added by Juan Francisco L.
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Specifically, focus on the peak labeled as 20.3 in the D12S391 locus and the two OLs (off-ladder peaks) in the SE33 locus. Show more…
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Topic 1: Reading an electropherogram The image below is the electropherogram from an individual (blue line) at four STR loci (D8S1179, D21S11, D7S820, and CSF1PO). Below each peak is the number of repeats for each allele. Given this information, answer the following questions and explain how you determined the answer: 1. For each of the four STR loci is the individual a heterozygote or homozygote AND how do you know? 2. Which of the STR has the longest DNA fragment AND how do you know?
Farhan A.
Madhur L.
3. (Total 2 points) Consider the following pedigree and microsatellite analysis. A. (1 points) What is the genetic basis of this trait (mode of inheritance)? B. (1 points) There are three DNA markers and the sizes are 250 bp, 220 bp, 200 bp. Explain which marker allele, if any, is linked to the trait of interest? 4. (Total 2 points) Mutations in several genes can cause the adult onset disorder shown in the pedigree below. Microsatellite markers, DM1 and DM2, are linked to two of the possible genes.
Adi S.
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