the following events in an aseptic transfer in order from first to last. Flame the loop for the first time. Label the sterile broth tube with the name of the bacteria you are using and your initials. Remove a loopful of bacterial culture from the bacterial culture tube. Flame the mouth of the bacterial culture tube and replace the cap. Insert the loop into the sterile broth tube. Remove the cap of the bacterial culture tube and flame the mouth of the tube. Remove the cap of the sterile broth tube and flame the mouth of the tube. Flame the loop for the second time. Flame the mouth of the sterile broth tube and replace the cap.
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Step 1: Label the sterile broth tube with the name of the bacteria you are using and your initials. Show more…
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Adi S.
Text: Select all of the TRUE statements regarding proper aseptic culturing technique? (Mark all that apply) a. When inoculating new media, all tubes and plates should be clearly labeled before any transfers are made. b. When flaming a loop, begin at the base, then slowly pull the loop through the flame until it is red hot. c. Blow gently on the sterilized inoculating loop to make sure it is cool enough to pick up the culture. d. After removing the cap of the culture tube, keep it in your hand pointed down to minimize the risk of contamination. e. When subculturing from a broth, the tube should be gently mixed before transfer.
Sri K.
You must inoculate one nutrient broth tube and two agar plates from an organism on an agar slant. Answer the following questions assuming you have completed the inoculations. Write the number on the line. A. What is the minimum number of times you needed to correctly flame the open end of a tube? The answer is the sum for all tubes, NOT per tube. B. What is the minimum number of times you needed to flame your inoculating loop? Again, the answer is the sum for the entire procedure. C. What is the minimum number of times you needed to set a tube's cap on the laboratory bench? And again, the answer is the sum for the entire procedure.
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