Question

19. If you move the microscope ruler to the right, how do you see the image move? a. to the right b. to the left c. toward you d. away from you e. none of the above 20. A microscope is parfocalized if when going from the low power objective to the high power objective all you need to adjust for focus is the fine adjustment knob. a. true b. false 21. The focal length (working distance) a. is the distance between the objective lens and the object when the objective is in focus b. Goes down as the magnification of the objective lens goes up c. a and b only d. a, b and c are correct 22. Refraction is the bending of light as it passes from a lens into the air. a. true b. false 23. Dyes are a. molecules that absorb part of the visible spectrum b. have a chromophore (colored part) and an auxochrome (attaches the dye to the substrate) c. often are positively charged, whereas the bacterial cell is negatively charged d. all the above e. none of the above 24. A simple stain a. uses one dye b. allows the viewer to see morphology (size, shape and structure) and arrangement of microbes. c. permits the differentiation of two groups of microbes d. a and b only e. a, b and c 25. The gram stain may give variable results because a. In an aging culture the cell wall begins to disintegrate and gram-positive cells may not fully retain the crystal violet-iodine complex b. Smears of bacterial colonies or organisms may be decolorized too long c. Some antimicrobial agents may change bacterial cell walls d. a and b e. a, b and c

19. If you move the microscope ruler to the right, how do you see the image move?
a. to the right b. to the left c. toward you d. away from you
e. none of the above

20. A microscope is parfocalized if when going from the low power objective to the high power objective all you need to adjust for focus is the fine adjustment knob.
a. true b. false

21. The focal length (working distance)
a. is the distance between the objective lens and the object when the objective is in focus
b. Goes down as the magnification of the objective lens goes up
c. a and b only
d. a, b and c are correct

22. Refraction is the bending of light as it passes from a lens into the air.
a. true b. false

23. Dyes are
a. molecules that absorb part of the visible spectrum
b. have a chromophore (colored part) and an auxochrome (attaches the dye to the substrate)
c. often are positively charged, whereas the bacterial cell is negatively charged
d. all the above
e. none of the above

24. A simple stain
a. uses one dye
b. allows the viewer to see morphology (size, shape and structure) and arrangement of microbes.
c. permits the differentiation of two groups of microbes
d. a and b only
e. a, b and c

25. The gram stain may give variable results because
a. In an aging culture the cell wall begins to disintegrate and gram-positive cells may not fully retain the crystal violet-iodine complex
b. Smears of bacterial colonies or organisms may be decolorized too long
c. Some antimicrobial agents may change bacterial cell walls
d. a and b
e. a, b and c

$19. If you move the microscope ruler to the right, how do you see the image move? a. to the right b. to the left c. toward you d. away from you e. none of the above 20. A microscope is parfocalized if when going from the low power objective to the high power objective all you need to adjust for focus is the fine adjustment knob. a. true b. false 21. The focal length (working distance) a. is the distance between the objective lens and the object when the objective is in focus b. Goes down as the magnification of the objective lens goes up c. a and b only d. a, b and c are correct 22. Refraction is the bending of light as it passes from a lens into the air. a. true b. false 23. Dyes are a. molecules that absorb part of the visible spectrum b. have a chromophore (colored part) and an auxochrome (attaches the dye to the substrate) c. often are positively charged, whereas the bacterial cell is negatively charged d. all the above e. none of the above 24. A simple stain a. uses one dye b. allows the viewer to see morphology (size, shape and structure) and arrangement of microbes. c. permits the differentiation of two groups of microbes d. a and b only e. a, b and c 25. The gram stain may give variable results because a. In an aging culture the cell wall begins to disintegrate and gram-positive cells may not fully retain the crystal violet-iodine complex b. Smears of bacterial colonies or organisms may be decolorized too long c. Some antimicrobial agents may change bacterial cell walls d. a and b e. a, b and c$

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