00:01
The question that is given over here, it says that what type of enzyme is used in our dna technology to split the specific sugar -pospate bond in each standard of dnawx.
00:20
Some of the options are given here.
00:22
The right answer for this question is optionally, the estates, the sugar phosphate or basically the prosper diastor bond.
00:44
So in order to split them, the estrange enzyme would be.
00:47
Helpful and then the next part of the question it says that dna replication is what dna replication is a semi -conservative the right answer for this question is option c semi -conservative the reason is that the dna molecule after replication it produces two exact copies of the original dna where each dna molecule has one strand from the original terenical one and another newly synthesized one.
01:36
And then the next part of the question, it says that the reaction of the dna replication catalyzed by the dna ligase is what? the right answer for this question is option c, that is the formation of phospho diastro bond with 3 prime oh of the one vokazaki fragment and 5 prime boss code of the mixed one of the lagging strand.
02:15
So, so so in dna replication, the main enzyme is dna polymerase.
02:21
It works in a 5 prime to 3 prime fashion.
02:29
So the leading strand is easily replicated.
02:31
Therefore, the lagging stand with things are a little tricky.
02:34
And here comes the concept of the okazaki fragments.
02:37
The lagging strand is replicated in the fragments.
02:40
And after all, the fragments of the newly synthesized dna produced, thus they all need to be joined.
02:47
Now, they are ligated by the enzyme called the fragment.
02:49
Dna ligates...