General questions (you can answer these questions before coming to the lab): You have run both non-denaturing (ND) and denaturing (D) samples of your purified fluorescent proteins on the SDS-PAGE gel. In which case(s) do you expect to see a different band pattern between the two samples (Y for yes pr N for no)? Briefly explain. Protein conformation Diff bands ND / D (Y or N) Explain Monomer Dimer Tandem dimer Tetramer What is the number of amino acids and the molecular weight of the in the GFP protein and DsRed monomers? (Include your source/reference) GFP protein: Source/reference: DsRed protein: Source/reference: 2 Page
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- Different bands (ND/D YorN) likely refers to the presence or absence of certain bands in a protein gel electrophoresis, indicating whether a protein is native (N) or denatured (D). - Monomer, dimer, tandem dimer, and tetramer refer to the different forms of Show more…
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