The PCR product from around 500 BP did the PCR walk
Added by Derrick K.
Step 1
These primers should be designed to amplify a small portion of the DNA sequence adjacent to the initial PCR product. Show more…
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Jenny W.
You used PCR to amplify the human insulin gene from cDNA template. The target sequence for this PCR reaction is approximately 600 base pairs (bp). To confirm that your PCR worked, you run the PCR products on gel and obtain the results shown below. The left lane contains DNA fragments of known sizes and the right lane contains your PCR products. Use these results to determine whether each of the following statements is true or false. The correct size target sequence was not amplified in this reaction. True or False? One of the primers failed to bind the cDNA template. True or False? One of the primers was able to bind at two locations in the cDNA template. True or False?
A researcher starts a PCR reaction with one molecule of chromosome 1 and an excess of primers that amplify a 400 bp PCR product from chromosome 1. Use the drawing described below and words to explain why the 400 bp PCR products do not form until the end of the third cycle of PCR (denature-anneal-extend is one PCR cycle). - Draw two cycles of PCR, being sure to indicate all 5' ends. - Delineate primers by color or line thickness. - How many 400 bp dsDNA PCR products will be synthesized by the end of the third PCR cycle?
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